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Table 1 Statistical evaluation of the results concerning the effects of ABCA1 on response to Curcumin

From: Overexpression of the ATP binding cassette gene ABCA1 determines resistance to Curcumin in M14 melanoma cells

p65     
Bonferroni's Multiple Comparison Test Mean Diff. t P value 95% CI of diff
   435nonsi vs 435nonsi Cur 1,393 0,2931 P > 0.05 -11.03 to 13.81
   435nonsi vs 435ABCA1si 12,22 2,57 P > 0.05 -0.2006 to 24.64
   435nonsi vs 435ABCA1si Cur 18,4 3,872 P < 0.01 5.985 to 30.82
bcl2     
Bonferroni's Multiple Comparison Test Mean Diff. t P value 95% CI of diff
   M14nonsi vs M14nonsi Cur -0,00009025 0,4383 P > 0.05 -0.0006282 to 0.0004477
   M14nonsi vs M14ABCA1si 0,0004488 2,18 P > 0.05 -0.00008914 to 0.0009868
   M14nonsi vs M14ABCA1si Cur 0,0009655 4,689 P < 0.001 0.0004275 to 0.001503
Survivin     
Bonferroni's Multiple Comparison Test Mean Diff. t P value 95% CI of diff
   M14nonsi vs M14nonsi Cur -0,00006799 0,1184 P > 0.05 -0.001568 to 0.001432
   M14nonsi vs M14ABCA1si 0,001349 2,349 P > 0.05 -0.0001513 to 0.002849
   M14nonsi vs M14ABCA1si Cur 0,002271 3,955 P < 0.01 0.0007706 to 0.003770
Apoptose     
Bonferroni's Multiple Comparison Test Mean Diff. t P value 95% CI of diff
   M14nonsi vs M14nonsi Cur 0,000869 0,005529 P > 0.05 -0.4193 to 0.4210
   M14nonsi vs M14ABCA1si -0,07965 0,5067 P > 0.05 -0.4998 to 0.3405
   M14nonsi vs M14ABCA1si Cur -0,688 4,377 P < 0.01 -1.108 to -0.2679
  1. Knocking-down the multidrug resistance gene alone already inhibits expression of the NFκB subunit p65 and the survival factors bcl2 and survivin and induces apoptosis in melanoma cells. However these effects are not statistically significant. Only the combination of knocking down ABCA1 together with simultaneous application of Curcumin results in a statistically highly significant response in terms of apoptosis.