Curcumin analogs efficiently inhibit xFANCD2 monoubiquitylation in Xenopus extracts. A) Inhibition of xFANCD2-Ub and xMRE11 phosphorylation by curcumin anlogs. IC50 values were determined using immunoblots of plasmid-activated extracts treated with a range of concentrations for each compound (0, 10, 25, 60, 150, 400, 1000, 3000 μM). A representative experiment is shown (out of 3 repeats). B) Structure of curcumin and monoketone analogs. C) Curcumin analogs do not affect RPA32 and H2AX phosphorylation. Activated extracts were treated with 1 mM each compound and phosphorylation of RPA32 and H2AX monitored by immunoblot.