Figure 4

Histological evaluation of Wnt-1 tumors of different SAFB1 genotype. A) Both SAFB1^+/+/Wnt-1 and SAFB1^+/-/Wnt-1 mice exhibit papillary, cribriform and squamous tumor type patterns. Representative pictures are shown (magnification 20×). B) Tumors found in the female (n = 10 per each genotype) mice were sectioned and stained with phospho H3 antibody. Representative pictures are shown (magnification 40×). The graph represents the quantification of phospho H3-positive cells/field. Error bars represent SEM. C). Wnt-1 tumours found in the female mice were sectioned and stained with caspase 3 antibody (n = 10 per each genotype). Representative pictures are shown (magnification 40×). The graph shows quantification of caspase 3-positive cells/field, with error bars representing SEM (p = 0.0742). Bars represent 100 μm.