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Figure 5 | Molecular Cancer

Figure 5

From: δ-Catenin promotes prostate cancer cell growth and progression by altering cell cycle and survival gene profiles

Figure 5

Changes in gene profiles of CWR22Rv-1 cells expressing δ-catenin when compared to that of cells expressing vector alone. A. RT2 Profiler™ PCR Array. CWR22Rv-1 cells with or without δ-catenin overexpression were subject to RT2 Profiler™ PCR arrays of apoptosis and cell cycle. Left panel shows the schematic illustration of one of the array outcomes. Right panel shows selected genes revealing over 2-folds upregulation. B. Selective, single real-time PCR analyses to compare RNA expression of cyclin D1, Bcl2L1 and HK2 in CWR22Rv-1 cells with or without δ-catenin overexpression. C. Changes in protein expression in CWR22Rv-1 cells with (+) or without (-) δ-catenin overexpression. Cells were lysed and proteins were separated by SDS-PAGE followed by Western blots using antibodies against proteins indicated on the right. After the blots were exposed using chemiluminence, the same blot was re-probed using mouse anti-actin to demonstrate protein loading control. Molecular weight markers in kDa are indicated on the left.

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Molecular Cancer

ISSN: 1476-4598