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Figure 1 | Molecular Cancer

Figure 1

From: Evaluation of bioluminescent imaging for noninvasive monitoring of colorectal cancer progression in the liver and its response to immunogene therapy

Figure 1

Characterization of the MC38Luc1 cell line. A.-Three independent MC38Luc clones were seeded in 96-well plates at different cell densities, and incubated with the luciferase substrate D-luciferin. Light emission from living cells in each well was immediately quantified using a CCD camera. The horizontal discontinuous line indicates the background light emission of an empty well. B. – The same procedure was used to quantify light emission from clone 1 (MC38Luc1) after maintenance in vitro for at least 40 passages. C. – Expression of luciferase was detected in MC38Luc1 cells at passage 30 by immunocytochemistry (right panel). Left panel corresponds to the parental MC38 cells. Magnification 400×. D. – MC38Luc1 or the parental MC38 cells (106 per mouse) were injected subcutaneously in the right hind flank of C57BL/6 mice (n = 10) to compare tumorigenicity. Tumor volumes achieved 3 weeks after cell implantation are shown. Error bars represent standard deviations.

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