Figure 6
Antitumor effect of IL-12 monitored by BLI. MC38Luc1 cells (5 × 105) were injected in the liver of C57BL/6 mice (n = 26). After verification of cell engraftment, the vector GL-Ad/RUmIL-12 was injected intravenously at 2.5 × 108 iu/mouse in half of the mice, and IL-12 expression was activated by mifepristone one week later. A. – Quantification of light emission before virus injection (day 2) and 7 days after initiation of IL-12 expression (day 17) in control (Co) and treated groups (IL-12). B. – Monitoring of tumor progression by BLI. Control group is represented as a dotted line. Three subgroups were defined among treated animals: non-responders (NR), mice with partial response (PR) and complete response (CR). Differences were statistically significant (p < 0.01) between Co and PR groups during days 20 to 30. Light emission from the CR group remained significantly lower until the end of the experiment. C. – Average tumor volume of the different groups one month after cell implantation, and at the time of sacrifice or spontaneous death. No evidence of tumor was observed in the CR group. Error bars represent standard deviations. * p < 0.05; ** p < 0.01.