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Figure 6 | Molecular Cancer

Figure 6

From: PRIMA-1MET induces nucleolar translocation of Epstein-Barr virus-encoded EBNA-5 protein

Figure 6

Fluorescence loss in photobleaching (FLIP) experiment of untreated DSRed-EBNA-5 expressing MCF7 cells show very high mobility of homogeneously distributed DSRed-EBNA5. Region of interest 1 (ROI 1) was bleached for 300 ms followed by recording of 10 consecutive images at 500 ms intervals. These cycles were repeated for 5 minutes. ROI 1 and 5, representing the total bleached area and a selected bleached subarea, showed rapid recovery of fluorescence. ROI 2, representing a remote non-bleached area in the nucleoplasm showed rapid homogeneous decrease of fluorescence. ROI 6 in adjacent cell showed no changes. ROI 3 and 4 in the nucleolus show two differently equilibrating compartment one faster (ROI 3) and one slower (ROI 4).

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