Figure 8
From: PRIMA-1MET induces nucleolar translocation of Epstein-Barr virus-encoded EBNA-5 protein
The PRIMA-1MET-induced nucleolar translocation of DSRed-EBNA-5 is reversible in the p53 -/- H1299 cells. The translocation was induced by 12 hours drug treatment followed by extensive washing with drug free medium. Time-lapse movie was recorded for 6 hours, one frame per minute. The fluorescence images are combined with phase contrast pictures to demonstrate the intact cellular morphology of the cells during the entire length of the experiment.