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Figure 2 | Molecular Cancer

Figure 2

From: Hexa-arginine enhanced uptake and residualization of selective high affinity ligands by Raji lymphoma cells

Figure 2

Binding of 111In radiolabeled SHAL (DvLPBaPPP) 2 LLDo and its hexa-arginine analog (DvLPBaPPP) 2 LArg 6 AcLLDo to Raji cells. Total 111In- (DvLPBaPPP)2LArg6AcLLDo bound to Raji cells (unwashed), solid squares; total 111In-(DvLPBaPPP)2LLDo bound to Raji cells (unwashed), open squares. Cell pellets containing 106 cells were resuspended in 150 μl 5%BSA/PBS buffer containing 0–25 ng of 111In labeled SHAL and incubated at RT for 1 hour. The samples were centrifuged to separate the cells from the supernatant and both were counted in a calibrated gamma well counter to quantify bound and unbound SHAL. Error bars are included for each data point, but in the majority of the cases the error is smaller than the data point and the error bar is not visible.

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Molecular Cancer

ISSN: 1476-4598