Figure 3
Fluorescent 3-D confocal microscopy of parent SHAL (DvLPBaPPP) 2 LLDo (top row) binding to live Raji cells compared to the hexa-arginine analog (DvLPBaPPP) 2 LArg 6 AcLLDo (middle row). Two mid-cell focal planes within the Raji cells are shown (left to right). Jurkat's cells treated with (DvLPBaPPP)2LArg6AcLLDo (left panel, bottom row) show minimal SHAL uptake. Lym-1 (right panel, bottom row) exhibits primarily cell surface membrane binding to Raji cells. The parent SHAL shows intracellular binding, while the hexa-arginine analog demonstrates not only marked cytoplasmic binding but also intranuclear targeting. DAPI (blue) is used as the nuclear stain and AlexaFlor 610 (red) demonstrates the location of SHAL in these merged sequential laser images.