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Table 1 Retention (residualization) of bound SHAL by Raji cells.

From: Hexa-arginine enhanced uptake and residualization of selective high affinity ligands by Raji lymphoma cells

 

pmoles SHAL Bound/106 cells

Percent SHAL Retained

SHAL

Unwashed

Washed

 

(DvLPBaPPP)2LLDo

0.568 ± 0.091

0.263 ± 0.000

46

(DvLPBaPPP)2LArg6AcLLDo

1.300 ± 0.038

0.876 ± 0.017

67

  1. Identical samples of cells used in experiments shown in Figure 2 were incubated as described in the Materials and Methods with 5.3 pmoles of 111In-labeled (DvLPBaPPP)2LLDo or (DvLPBaPPP)2LArg6AcLLDo and then washed two times with BSA/buffer. The cell pellets were then counted to provide estimates of SHAL remaining bound after washing.