Figure 4
Effect of ritonavir on DNA synthesis of MDAH-2774 cells. MDAH-2774 and normal human fibroblasts were seeded at 106 cells and grown serum-free medium for 48 h for cell cycle synchronization. The cells were then treated with either vehicle DMSO (A) or 5 μM ritonavir (B) or 20 μM ritonavir (C). Cells were further incubated in presence of serum for 24 h followed by fixation, staining and cytometric analysis using Cell cycle phase determination kit (Cayman chemical company, Ann Arbor, MI).