Effect of AKT siRNA, ritonavir and modulators of AKT signaling on the growth of ovarian cancer cell lines. Cells were cultured for indicated time points with various concentrations of the ritonavir. Cell growth was assessed by CCK-8 cell proliferation assay method and is expressed as a percentage of control (DMSO treated cells) and represents the mean of triplicate cultures. (R-ritonavir) A. Effect of ritonavir, siRNA and in combination; B. Effect of ritonavir and siRNA in presence of IGF-1. C. Effect of ritonavir in the presence of AKT inhibitor LY294002.