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Figure 4 | Molecular Cancer

Figure 4

From: Identification of the B-Raf/Mek/Erk MAP kinase pathway as a target for all-trans retinoic acid during skin cancer promotion

Figure 4

ATRA suppresses TPA induced phosphorylation of B-Raf, Mek1/2, and Erk1/2. A. IHC staining of 3 week treated skin. Paraffin sections of mouse skin treated with Acetone (Control), TPA, TPA+ATRA, and ATRA alone were probed with pB-Raf, pMek1/2, and pERK1/2 antibodies followed by an Alexa-546-labeled secondary antibody. Sections were counterstaining with DAPI. All panels were photographed at 600× magnification. B. Epidermal lysates were prepared in RIPA buffer from SENCAR mouse skin treated with Acetone (Con), TPA, TPA+ATRA (T+A), or ATRA alone for 10 weeks. Total protein (15 μg per well) from pooled samples (n = 5) was run on 10% SDS-PAGE and probed with antibodies for total B-Raf, pB-Raf, total Mek1/2, pMek1/2, total Erk1/2, pERK1/2, and β-actin. C. Epidermal thickness during the time course. Thickness was measured from digital micrographs of H&E stained skin sections as described in Methods. The symbols and error bars indicate the mean of 10 measurements ± SEM.

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