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Figure 2 | Molecular Cancer

Figure 2

From: siRNA-based targeting of antiapoptotic genes can reverse chemoresistance in P-glycoprotein expressing chondrosarcoma cells

Figure 2

Cell surface expression of P-glycoprotein and doxorubicin uptake and efflux in chondrosarcoma cells. (A) Cell surface P-glycoprotein expression was detected by flow cytometric analysis. Cell surface P-glycoprotein staining was positive in two chondrosarcoma cells. (IgG: isotype IgG control, PG: P-glycoprotein antibody) (B) Doxorubicin uptake was measured in two chondrosarcoma cells. Doxorubicin uptake increased as doxorubicin concentration was increased in the two chondrosarcoma cell lines. (C) Functional assay of P-glycoprotein was performed after doxorubicin washout. Doxorubicin levels were measured at 1, 6 and 24 hours after washout after 1 hour treatment. Doxorubicin levels decreased in a time dependant manner.

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Molecular Cancer

ISSN: 1476-4598