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Figure 4 | Molecular Cancer

Figure 4

From: The role of cellular oxidative stress in regulating glycolysis energy metabolism in hepatoma cells

Figure 4

Reduction of cellular ROS decreases glycolytic activity. (a) Cellular ROS was decreased either by ROS scavenging or by MnSOD transfection. (b) LDH activity was inhibited by reduction of cellular ROS. SMMC-7721 cells transfected with a void vector (SOD-) or with a vector expressing SOD (SOD+) were incubated for 24 h in the presence or absence of 5 mM α-LA. ROS levels (a) were determined by DCF fluorescence, and LDH activity levels (b) were measured as described in "Materials and methods". Results were expressed relative to control SMMC-7721 cells (SOD-) in the absence of α-LA treatment. * P < 0.05 versus control SMMC-7721 cells (SOD-) in the absence of α-LA treatment. The values represent the means ± S.D. from three separated experiments.

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