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Figure 6 | Molecular Cancer

Figure 6

From: The role of cellular oxidative stress in regulating glycolysis energy metabolism in hepatoma cells

Figure 6

Reduction of ROS inhibits the growth of tumor cells and xenograft tumors. (a) Reduction of ROS inhibits SMMC-7721 hepatoma cells growth. SMMC-7721 hepatoma cells were administrated with 5 mM α-LA or transfected with MnSOD gene (SOD-7721 cells) to downregulate cellular ROS. Cells were harvested at day 1, 2, 3 and 4. Cell growth was monitored by live cell counting using trypan blue exclusion method. (b) Comparison of different doses of α-LA on the growth of SMMC-7721 hepatoma cells and L02 immortalized normal liver cells. Cells were treated with 1, 2.5, and 5 mM α-LA, and harvested at 3 days after treatment. Cell growth was monitored by live cell counting using trypan blue exclusion method. (c) α-LA induces apoptosis in SMMC-7721 human hepatoma cells. Cells were cultured for 3 days with 1 mM, 2.5 mM and 5 mM α-LA. Apoptosis was evaluated by TUNEL as described in methods. (d) Tumor sizes in nude mice bearing human tumor xenografts. Nude mice were injected with SMMC-7721 cells transfected with a void vector or a vector expressing SOD (SOD-7721) as described in the methods. Tumor sizes were measured in three directions every 5 days. (e) Tumor masses in nude mice bearing human tumor xenografts. After 5 weeks, mice were killed and tumor masses were weighted. For cell experiments, the values represent the means ± S.D. from three separated experiments. * p < 0.05 compared with SMMC-7721 cells. For animal experiments, results were expressed as means ± S.D., n = 10 per group. * p < 0.05, compared with mice bearing SMMC-7721 cells.

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