Figure 6From: The role of cellular oxidative stress in regulating glycolysis energy metabolism in hepatoma cellsReduction of ROS inhibits the growth of tumor cells and xenograft tumors. (a) Reduction of ROS inhibits SMMC-7721 hepatoma cells growth. SMMC-7721 hepatoma cells were administrated with 5 mM α-LA or transfected with MnSOD gene (SOD-7721 cells) to downregulate cellular ROS. Cells were harvested at day 1, 2, 3 and 4. Cell growth was monitored by live cell counting using trypan blue exclusion method. (b) Comparison of different doses of α-LA on the growth of SMMC-7721 hepatoma cells and L02 immortalized normal liver cells. Cells were treated with 1, 2.5, and 5 mM α-LA, and harvested at 3 days after treatment. Cell growth was monitored by live cell counting using trypan blue exclusion method. (c) α-LA induces apoptosis in SMMC-7721 human hepatoma cells. Cells were cultured for 3 days with 1 mM, 2.5 mM and 5 mM α-LA. Apoptosis was evaluated by TUNEL as described in methods. (d) Tumor sizes in nude mice bearing human tumor xenografts. Nude mice were injected with SMMC-7721 cells transfected with a void vector or a vector expressing SOD (SOD-7721) as described in the methods. Tumor sizes were measured in three directions every 5 days. (e) Tumor masses in nude mice bearing human tumor xenografts. After 5 weeks, mice were killed and tumor masses were weighted. For cell experiments, the values represent the means ± S.D. from three separated experiments. * p < 0.05 compared with SMMC-7721 cells. For animal experiments, results were expressed as means ± S.D., n = 10 per group. * p < 0.05, compared with mice bearing SMMC-7721 cells.Back to article page