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Figure 5 | Molecular Cancer

Figure 5

From: Identification of novel Notch target genes in T cell leukaemia

Figure 5

Identification of direct notch targets using a GSI washout experiment. Jurkat cells were treated with 10 uM GSI (or DMSO (untreated)) for 48 hours to accumulate cell surface Notch before washing to permit Notch signalling. After washing, cells were treated with 20 uM cyclohexamide (CHX) or ethanol (vehicle control) to inhibit protein synthesis. After 4 hrs, RNA was isolated and cDNA made for real-time PCR analysis of known Notch target genes (A) and novel Notch target genes (B). Expression values were calculated using cDNA from untreated cells as the calibrator sample. * represents p < 0.01 versus untreated cells (effect of Notch inhibition). ** prepresents p < 0.01 versus GSI-treated cells (effect of Notch signalling). *** represents p < 0.01 versus washout cells (effect of cyclohexamide treatment).

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