Figure 8

Analysis of Notch-induced VEGF, ID1 and GIMAP5 expression. (A) Supernatants from GFP-alone, N1ΔE, or N3ΔE-transduced Jurkat or CEM cells were used for VEGF ELISAs to analyse secreted VEGF protein levels in response to ectopic Notch. Supernatants of CEM cells transduced with DN-MAML were also analysed to show the contribution of endogenous Notch transcriptional activity on VEGF expression. * represents p < 0.05 versus GFP-alone-transduced cells (B) Jurkat cells were used for a GSI washout assay and ID1 and GIMAP5 gene expression analysed over 4 hrs following washout using cDNA from untreated cells as the calibrator sample. (C) Western blot analysis of ID1 and GIMAP5 expression using protein extracts from Jurkat cells treated with a dose range of GSI IX.