Pancreatic cancer cell lines PANC-1 and S2-013 show divergent HO-1 expression levels. mRNA extractions from cells for QRT-PCR and cell lysates for Western blot were prepared from untreated cells and cells treated with gemcitabine. (1A) No treatment of PANC-1 cells displayed high native mRNA expression of HO-1, whereas HO-1 expression was only at levels of detection in S2-013. Treatment with gemcitabine increased mRNA expression of HO-1 in PANC-1 cells but did not alter the expression level in S2-013 cancer cells. (1B) Protein analyses in resting condition revealed high native expression of HO-1 in PANC-1 but faint expression in S2-013. Under gemcitabine conditions protein expression of HO-1 was slightly induced in PANC-1 cell line but did not alter HO-1 protein expression in S2-013 cell line.