Depletion of iron increases susceptibility to chemotherapy in vivo and in vitro. Cell growth was assessed 72 h after application of gemcitabine via MTT assay. (6A) Implementation of DFO resulted in dose dependent profound growth inhibition of both cell lines. (6B) Primary pancreatic tumor weights were assessed 4 weeks after orthotopic tumor implantation of S2-013 cancer cells in nude mice (n = 60; 15 mice per group). DFO plus gemcitabine reduced the tumor growth significantly (tested for S2-013 cell line only).