Figure 1

TRAIL induced caspase activation in PC-3 cells does not lead to apoptosis. A. PC-3 cells were treated with TRAIL (25 ng/ml) for the indicated times. Caspase (DEVDase) activity was measured using a fluorometric assay as described in materials and methods with activity relative to control cells. B. Cells were treated with TRAIL (25 ng/ml) for 3 hours. The expression levels of caspases -3, -8, and -9, and their cleaved products were detected by Western blotting. Data shown is representative of a minimum of three independent experiments. C. Apoptosis (%) was assessed by propidium iodide DNA staining and flow cytometry after 6 – 24 hours of TRAIL treatment in PC-3 cells. Treatment of TRAIL in combination with resveratrol was included at all time points as a positive control ((+)ve con) for apoptosis induction.