Skip to main content
Figure 3 | Molecular Cancer

Figure 3

From: Effects of cIAP-1, cIAP-2 and XIAP triple knockdown on prostate cancer cell susceptibility to apoptosis, cell survival and proliferation

Figure 3

Combined IAP knockdown is required to sensitise PC-3 cells to TRAIL-induced death. Cells were transfected with siRNA targeting cIAP-1 (0.5 nM), cIAP-2 (0.5 nM), XIAP (0.5 nM), or combinations thereof (as indicated on x-axis), and corresponding non-targeting control siRNA (siCon) to a final concentration of 1.5 nM for each group. 24 hour post-transfection cells were treated for a further 24 hours with TRAIL (25 ng/ml, A and B), Etoposide (10 μM, C and D), or Tunicmycin (2 μg/ml, E and F). Viability (A, C, E) and Apoptosis (B, D, F) were assessed by propidium iodide DNA staining and subsequent flow cytometric analysis. Values are expressed as mean +/- SD. *Significantly different from cells transfected with non-targeting control (siCon) followed by treatment (TRAIL/Etop/Tm) at p < 0.01.

Back to article page

Molecular Cancer

ISSN: 1476-4598