Figure 7

Western blot analysis of PC protein patterns of paired tumor and non-cancerous tissues from patients #6–10. Western blotting (panel A) and image analysis (panel B) were performed as described in Methods. Normalized PC response represented PC image density normalized to α-tubulin image density. The non-cancerous tissue of patient #8 had a high interfering background with no discernable PC band in the blot image, which was not quantified. The cancer tissue of patient #10 had a very intense PC band, which along with the PC band of the non-cancerous tissue was quantified using the blot image with 2 min of film exposure, as was the case for the α-tubulin band of all tissues. PC band for the rest of tissues was quantified using the same blot but with 17 min of film exposure. N: non-cancerous; C: cancer; ND: not determined. The data shown is representative of two separate blot analyses.