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Table 6 Forward and reverse primer probes used for RT-PCR of mitochondrial dehydrogenases, pyruvate carboxylase, and glutaminaseA

From: Altered regulation of metabolic pathways in human lung cancer discerned by 13C stable isotope-resolved metabolomics (SIRM)

Gene/accession no

Forward sequence

Reverse sequence

FH/NM_000143.2

TCTGGTCCTCGGTCAGGTCTG

GACAGTGACAGCAACATGGTTCC

GLS/NM_014905

GCACAGACATGGTTGGTATATTAG

AGAAGTCATACATGCCACAGG

IDH3/NM_005530.2

CAACTGCCCCTTCTCCTATCCC

AGCCCAAGCCTAAGCCCAAG

MDH2/NM_005918.2

CGGAGGTGGTCAAGGCTAAAG

CAGCGGTGTGGAGAAGTAGG

OGDH/NM_002541.2

GTGAGAATGGCGTGGACTAC

CGATTGATCCTGCGGTGATAC

PC/NM_022172

GCGTGTTTGACTACAGTGAG

TCTTGACCTCCTTGAACTTG

SDH/NM_004168.2

CATCGCATAAGAGCAAAGAAC

CCTTCCGTAATGAGACAACC

18S/NR_003286

ATCAGATACCGTCGTAGTTCC

CCGTCAATT CCTTTAAGTTTCAG

  1. A The abbreviations are as in Table 5.
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Molecular Cancer

ISSN: 1476-4598