Skip to main content

Advertisement

Figure 1 | Molecular Cancer

Figure 1

From: Survivin counteracts the therapeutic effect of microtubule de-stabilizers by stabilizing tubulin polymers

Figure 1

BPR0L075 induces over-expression of survivin in human KB and HONE-1 cancer cells. (A) Cells were treated with 8 nM of BPR0L075 and 10 nM of colchicine for 6 h, 12 h and 24 h. Cell lysate were extracted and proteins were resolved by SDS-PAGE. Western blot analysis was performed as described in Materials and Methods. (B) KB cells were treated with 8 nM of BPR0L075 for 24 h and total RNA was extracted. Level of survivin mRNA was determined by RT-PCR. GAPDH was included for the use of semi-quantization. Relative band intensity was shown. (C) KB cells were incubated with 8 nM of BPR0L075 for 24 h with/without pifithrin-α pre-incubation. Cell lysate were extracted and proteins were resolved by SDS-PAGE. Western blot analysis was performed as described in Materials and Methods. Relative band intensity was shown. (D) KB-L30 cells over-expresses survivin. Total cell lysate of KB and K-L30 cells was extracted and proteins were resolved by SDS-PAGE. Western blot analysis was performed as described in Materials and Methods. Relative band intensity was shown. (E) Total mRNA of KB and KB-L30 cells was extracted. Level of survivin mRNA was determined by real-time PCR. Relative survivin to GAPDH ratios were shown. A statistically significant difference in the level of survivin mRNA between KB and KB-L30 cells is denoted by "*", (p < 0.001).

Back to article page