Figure 1

Kinome-wide siRNA screen to find HH pathway regulatory kinases in NSCLC A549 cells. A, Identification of GLI1 cascade activated NSCLC cell lines. The mRNA expression level of GLI1 in the eight NSCLC cells was measured with quantitative RT-PCR. Data were normalized to internal β-actin expression and are presented as the mean and SD of three independent experiments. B, Establishment of stable A549 cell lines harboring GLI regulatory β-lactamase reporter gene activity. The A549-GLI cells were treated with siRNAs as a control, GLI1, GLI2, GLI3 or PLK1 to confirm that β-lactamase was under the control of GLI1 transcription factor. PLK1-siRNA was used as a negative control to confirm that cytotoxicity did not affect reporter activity. Data are the mean and SD of three independent experiments. C, Results of the kinome-wide siRNA screen composed of about 500 protein kinases. The GLI reporter activity was shown as a percentage normalized to control-siRNA treated cells. **, P ≤ 0.01, compared with control.