Figure 2
From: Inhibition of p70S6K2 down-regulates Hedgehog/GLI pathway in non-small cell lung cancer cell lines
p70S6K2-silencing by siRNA down-regulates GLI regulatory reporter gene. A, mRNA-silencing level of p70S6K2 by siRNA. mRNA expression levels of p70S6K2 in the control- or p70S6K2-siRNA treated cells were measured 48 hr after siRNA transfection by quantitative RT-PCR. B, Effect of p70S6K2-silencing on GLI reporter gene activity. GLI regulatory β-lactamase activity was measured 72 and 96 hr after siRNA transfection. The value was normalized to cell number. C, Effect of p70S6K2-silencing on cell viability. Cell viability was measured 72 and 96 hr after siRNA transfection as described in the materials and methods. Data are the mean and SD of three independent experiments. **, P ≤ 0.01, compared with control.