Figure 3From: Inhibition of p70S6K2 down-regulates Hedgehog/GLI pathway in non-small cell lung cancer cell linesp70S6K2-silencing by siRNA decreases or increases expression of endogenous GLI1 regulatory genes. A, Cyclin D1 mRNA expression level. B, γ-catenin mRNA expression level. mRNA expression levels of GLI1-regulatory genes were measured 48 hr after siRNA transfection by quantitative RT-PCR in A549 cells treated with GLI1-siRNA or p70S6K2-siRNA. Data were normalized to internal β-actin expression and are presented as the mean and SD of three independent experiments. *, P ≤ 0.05; **, P ≤ 0.01 compared with control.Back to article page