Figure 5
From: Inhibition of p70S6K2 down-regulates Hedgehog/GLI pathway in non-small cell lung cancer cell lines
GLI reporter gene activity reduction by PIK3CA-inhibition. A, Effect of PI3K-silencing by siRNA on GLI regulatory reporter gene. GLI regulatory reporter gene activity was measured by β-lactamase assay 72 hr after siRNA transfection (PLK1, GLI1 and PIK3CA) into A549-GLI cells. PLK1-siRNA was treated as a negative control to confirm that cytotoxicity did not affect reporter activity. GLI1-siRNA was used as a positive control. B, Effect of PI3K-inhibition by LY294002 on GLI regulatory reporter gene. The A549-GLI cells were treated with PI3K inhibitor, LY294002 (90 μM) and SMOH inhibitor KAAD-cyclopamine (800 nM) respectively. β-lactamase activity was measured 12 hr after treatment. Data are the mean and SD of three independent experiments. **, P ≤ 0.01 compared with control.