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Figure 2 | Molecular Cancer

Figure 2

From: PRL-3 overexpression in epithelial cells is induced by surrounding stromal fibroblasts

Figure 2

Immunostaining of PRL-3 (Abcam, Cambridge, UK) in DLD-1 cells cultured in DMEMF12 + 10% FBS (A), CM from foreskin fibroblasts (B), CM from normal colonic fibroblasts (C), CM from CAFs from a primary tumour (D), CM from CAFs from liver metastasis (E). Briefly, cells were fixed 5 min. in ice cold methanol, washed in PBS and incubated for 1 hour in blocking buffer 1:5 in PBS (horse serum and goat serum, 1:1 v/v). Then, overnight incubation with rabbit polyclonal antibody against PRL-3 (Sigma Aldrich). After washing steps, 1 hour incubation with Goat anti rabbit IgG (H+L) FITC conjugated (Jackson).

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