Figure 5

Western blot analysis of poly (ADP-ribose) polymerase (PARP) cleavage in cell lysates obtained from NCI-H460 cultured for 24 to 30 hrs after infection with ReoT3D (MOI = 20) in the absence or presence of gemcitabine (1 μM), paclitaxel (1 μM) or vinblastine (0.1 μM). Shown are the full-length PARP (116 kDa) and the larger fragment (89 kDa) of apoptotically cleaved products, as well as β-actin used as a loading control. Results are representative of 2 separate experiments.