Figure 2From: Breast cancer cells stimulate osteoprotegerin (OPG) production by endothelial cells through direct cell contactInvolvement of the VEGF receptor VEGFR2 in OPG mediated endothelial tube formation. HuDMECs were cultured on growth factor reduced matrigel for 8 hours and branch point number counted as described in materials and methods. (a) Untreated control, (b) VEGF 10 ng/ml, (c) VEGF 10 ng/ml and VEGFR2 neutralising antibody (1 μg/ml), (d) OPG 100 ng/ml, (e) OPG 100 ng/ml and VEGFR2 neutralising antibody (1 μg/ml). (f) Quantification of tube formation through measurement of branch point number. Data represented as mean ± S.E.M. from three independent experiments performed in triplicate. ***, p < 0.001 compared to VEGF in combination with VEGFR2 antibody; NS, no significant difference.Back to article page