Figure 5From: 14-3-3σ gene silencing during melanoma progression and its role in cell cycle control and cellular senescence14-3-3σ induces cellular senescence in melanoma cells. (a) Expression of senescence marker SA-β-Gal in untransduced, vector control or 14-3-3σ cDNA transduced SK-Mel-147 melanoma cells. (b) SK-Mel-19 melanoma cells were exposed to 3.0 μg/ml adriamycin for indicated time points to induce cellular senescence. Induction of p53, 14-3-3σ and SA-β-Gal was analyzed by immunoblotting or immunohistochemistry in untransduced, vector control or 14-3-3σ shRNA (si14-3-3B4) transduced cells.Back to article page