Semi-quantitative Western blot was performed as described in Material and Methods. Protein expression levels of Prim-MEF were arbitrarily set to 1.0. Glycolytic and mitochondrial enzymes do not show upregulation at the protein level in immortalized or transformed cell populations. Two enzymes involved in cellular NADH metabolism, MDH1 and NAMPT showed increased protein levels.
*Indexed on Ras-LP (1.0): protein levels in Prim-MEF were extremely low and could not be reliably quantified (nd: not detectable).