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Table 2 Protein expression levels in primary, immortalized and tumorigenic cell populations

From: Increased OXPHOS activity precedes rise in glycolytic rate in H-RasV12/E1A transformed fibroblasts that develop a Warburg phenotype

  Prim-MEF Imm-MEF TBX2-MEF RAS-LP RAS-HP RAS-TUM
Glycolysis       
Aldolase 1.0 1.1 1.1 1.0 1.0 1.1
GAPDH 1.0 0.9 1.0 1.4 1.4 1.5
Pyruvate kinase 1.0 0.8 0.8 1.0 1.1 1.0
LDH-A 1.0 0.8 0.8 1.0 1.1 1.2
OXPHOS       
Porin 1.0 1.3 1.3 1.4 0.9 1.0
B39 1.0 0.7 0.7 1.3 1.5 1.5
PDH-E2 1.0 1.0 1.2 1.2 1.0 1.2
PDH-E1a 1.0 1.0 1.1 1.3 1.4 1.2
NAD metabolism       
cMDH 1.0 0.6 1.2 2.4 3.3 2.9
mMDH 1.0 0.9 0.9 1.3 1.1 1.1
NAMPT* nd nd nd 1.0 1.9 2.6
  1. Semi-quantitative Western blot was performed as described in Material and Methods. Protein expression levels of Prim-MEF were arbitrarily set to 1.0. Glycolytic and mitochondrial enzymes do not show upregulation at the protein level in immortalized or transformed cell populations. Two enzymes involved in cellular NADH metabolism, MDH1 and NAMPT showed increased protein levels.
  2. *Indexed on Ras-LP (1.0): protein levels in Prim-MEF were extremely low and could not be reliably quantified (nd: not detectable).