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Figure 1 | Molecular Cancer

Figure 1

From: Elongation Factor 1 alpha interacts with phospho-Akt in breast cancer cells and regulates their proliferation, survival and motility

Figure 1

Interaction between EF1α and Akt in SkBr3 cells. SkBr3 whole cell lysates and lysates of SkBr3 cells subjected to anti-pAkt (Ser473) IP in saturating conditions to deplete the active kinase were immunoprecipitated with anti-pAkt or anti-Akt1/Akt2 antibody, respectively. IPs were separated in two equal amounts and subjected to SDS-PAGE and western blotting using anti-Akt1/2 (panel b) and anti-pAkt (panel c) antibodies to distinguish pAkt and unphosphorylated Akt and to avoid superimposition of adjacent bands and background interference. Levels of associated EF1α were also detected in both membranes. Western blots show: (a) expression of pAkt and EF1α in whole cell and p-Akt-depleted lysates. Expression of β-Actin was used as loading control; expression of total Akt (Akt1/Akt2) and EF1α (b) or of pAkt and EF1α (c) in the anti-pAkt and in the anti-Akt (Akt1/Akt2) IPs from p-Akt-depleted SkBr3 cell lysate. In the anti-Akt1/2 IPs obtained after pAkt depletion, levels of residual pAkt (panel c) were negligible.

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Molecular Cancer

ISSN: 1476-4598