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Figure 2 | Molecular Cancer

Figure 2

From: Elongation Factor 1 alpha interacts with phospho-Akt in breast cancer cells and regulates their proliferation, survival and motility

Figure 2

(a) Western blotting of HCC1937 cell lysates and anti-Akt2 or anti-Akt1 IPs by anti-EF1α, anti-pAkt Ser473, anti-Akt1 and anti-Akt2 antibodies. Selective inhibitors of pAkt2 and pAkt1/2 were used to reduce the concentration of active pAkt. (b) Western blot shows expression of pAkt, Akt1, Akt2 and EF1α in untreated and pAkt1/2 inhibitor-treated SkBr3 cell lysate (lanes 1 and 2) and in anti-pAkt (from untreated cell lysates) and anti-Akt1/2 IPs (from pAkt inhibitor-treated cells) (lanes 3 and 4). (c) Western blot shows co-immunoprecipitation of HA-tagged pAkt1 and EF1α in SkBr3 cells transfected with HA-tagged Myr-Akt1 (lane 2) or T308A/S473A Akt1 (lane 3) expression vector. Lysate of SkBr3 wild type cells (lane 1) was used as control.

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