Figure 4From: Elongation Factor 1 alpha interacts with phospho-Akt in breast cancer cells and regulates their proliferation, survival and motilityEffect of EF1α expression on pAkt, Akt1 and Akt2 levels. a) Western blot shows levels of the indicated proteins (including β-actin for normalization) in HCC1937 cells 24 h after transfection with CTRLsiRNA or EF1α siRNAs; b) Western blot shows expression of pAkt in anti-Akt1 or anti-Akt2 IPs of CTRL or EF1α siRNA-transfected HCC1937 cells; c) Western blot shows pAkt and EF1α expression in lysate or IPs (anti-FLAG or anti-EF1α) of SkBr3 cells transfected with FLAG-PDK1.Back to article page