Figure 5From: Elongation Factor 1 alpha interacts with phospho-Akt in breast cancer cells and regulates their proliferation, survival and motilityColony formation of HCC1937 cells transfected with EF1α siRNA and/or treated with pAkt inhibitors. HCC1937 cells were transfected with 100 nM of EF1α or CTRLsiRNA and 24 h later counted and plated in 60-mm diameter dishes (3,000 cells for dish). For the co-treatment with siRNAs and the Akt inhibitor, transfected cells were plated in the presence of the Akt inhibitor (final concentration of 7 μM). After 14 days, cells were fixed and stained with crystal violet. Results are representative of three independent experiments with triplicate counts.Back to article page