Transcriptional activation of the Lats1 tumor suppressor gene in tumors of CUX1 transgenic mice

Table 1 Lats1 is identified as a putative target of p110 CUX1 in 8 cell lines

Genome-wide location analysis was performed using a panel of 8 human cell lines of various cell types: breast tumor (Hs578T), cervical carcinoma (HeLa), kidney epithelial (HEK293), myeloid leukemia (K562, HEL), and B cell lymphoma (Ramos, U266, RPMI8266). For each cell line, hybridization experiments were done in triplicate using three independent ChAP-enriched and input DNA samples (8 cell lines × 3 hybridizations = 24). ChAP-enriched DNA regions associated with p110 CUX1 were purified and labeled with Cy5 dye. Equal amounts of Cy5-labeled ChAP products and Cy3-labeled input DNA samples were co-hybridized onto a human genomic DNA microarray containing the region spanning 800 bp upstream and 200 bp downstream of transcription start sites of 18,660 human genes. P values observed for the Lats1 promoter are shown. The data is taken from [35].

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