Figure 1

Site-directed targeting of NH2-truncated p73 mRNAs by LNA gapmers. (A) Structure of the human TP73 gene. Exons are shown as boxes and structured according to the domain: transactivation domain (blue); exon 3B-derived coding sequence (purple); DNA-binding domain (red); oligomerization domain (yellow); COOH terminus (green). C-terminal splice variations are indicated. The transcriptional start sites of the two promoter regions (TA-promoter, ΔN-promoter) are marked by arrows. Aberrantly spliced transcripts regulated by the TA-promoter are labeled ΔEx2, ΔEx2/3, and ΔN'. The unique 5' untranslated region of the ΔN transcript is colored grey. (B) Binding sites of gapmer ASOs in different DNp73 transcripts. ASO-115/ΔEx2p73, ASO-116/ΔEx2/3, and ASO-185/451 directed against ΔN'p73 and ΔNp73 mRNA. LNA bases are in bold.