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Figure 2 | Molecular Cancer

Figure 2

From: Antisense gapmers selectively suppress individual oncogenic p73 splice isoforms and inhibit tumor growth in vivo

Figure 2

Efficacy and specificity of ASOs for endogenously upregulated p73 transcripts. (A) Quantitative RT-PCR of endogenous expression of TAp73 and amino-truncated p73 transcripts relative to p53 and p63 in HEK293 cells. The broken line indicates the baseline level of DNp73 transcripts. Cells were transfected with 250 nM of ASO-115 (B, F), ASO-116 (C, G), and ASO-185/451 (D, E, and H). Antisense effects on transcript levels were quantitated by real-time PCR at indicated time points after treatment. Fold expression was calculated after normalization with RPS9 relative to non-specific control-ASO (nsc). Averages and standard deviations of at least three independent experiments are shown.

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Molecular Cancer

ISSN: 1476-4598