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Figure 4 | Molecular Cancer

Figure 4

From: Vesnarinone downregulates CXCR4 expression via upregulation of Krüppel-like factor 2 in oral cancer cells

Figure 4

KLF2 binding to the CXCR4 promoter via a CACCC site. (a) ChIP assay. B88 cells were treated with or without vesnarinone for 48 h, or were transfected with pcDNA3 or pcDNA3-KLF2. Equal aliquots of isolated chromatin DNA were subjected to immunoprecipitation with rabbit KLF2 antibody, and the DNA fragments associated with specific immunoprecipitates were purified and used as templates for the PCR in order to amplify the CXCR4 promoter sequences containing the CACCC/KLF2 binding site (-401 to -142). (b) EMSA. Ten micrograms of recombinant GST or GST-KLF2 protein were incubated in the biotinylated CXCR4 promoter containing the CACCC site with or without an excess volume (200 fold) of non-labeled wild type probe (Cold WT Pr) or that of non-labeled mutated probe (Cold MU Pr).

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