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Figure 3 | Molecular Cancer

Figure 3

From: NC2213: a novel methionine aminopeptidase 2 inhibitor in human colon cancer HT29 cells

Figure 3

Expression of MetAP2 and Src in HT29 cells. A, expression of MetAP2; B, expression of Src. Cells were treated with NC2213 at various concentrations for 48 hours. Equal amounts of proteins were loaded on to each lane, subjected to SDS-PAGE, transferred to nitrocellulose membrane, and blotted with anti-MetAP2, anti-pp60c-src, anti-phospho-Src (Tyr527), anti-phospho-Src (Tyr416), as described under Materials and Methods. β-Actin was used as a loading control. The data presented as the representative of at least three separate experiments.

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