Expression of MetAP2 and Src in HT29 cells. A, expression of MetAP2; B, expression of Src. Cells were treated with NC2213 at various concentrations for 48 hours. Equal amounts of proteins were loaded on to each lane, subjected to SDS-PAGE, transferred to nitrocellulose membrane, and blotted with anti-MetAP2, anti-pp60c-src, anti-phospho-Src (Tyr527), anti-phospho-Src (Tyr416), as described under Materials and Methods. β-Actin was used as a loading control. The data presented as the representative of at least three separate experiments.