Skip to main content
Figure 4 | Molecular Cancer

Figure 4

From: Enhanced sensitivity of celecoxib in human glioblastoma cells: Induction of DNA damage leading to p53-dependent G1 cell cycle arrest and autophagy

Figure 4

Effect of celecoxib on p21 mRNA expression and protein localisation. Cells were synchronised in serum-free medium for 48 hours and then treated with celecoxib (8 and 30 μM) in medium containing 10% FBS for 18 hours. Total RNA was extracted from celecoxib-treated cells, followed by RT-PCR using p21 primers. p21 mRNA expression is presented normalised over GAPDH of same samples (A, n = 3–5). Representative images of celecoxib-treated cells immunostained with anti-p21 FITC and DAPI are shown (B, n = 3).

Back to article page

Molecular Cancer

ISSN: 1476-4598