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Figure 5 | Molecular Cancer

Figure 5

From: Enhanced sensitivity of celecoxib in human glioblastoma cells: Induction of DNA damage leading to p53-dependent G1 cell cycle arrest and autophagy

Figure 5

Effect of celecoxib on cell apoptosis. Cells were treated with celecoxib (8 and 30 μM) for 72 hours. In some cases, U87MG cells were pre-treated with PFT (25 μM) prior to celecoxib treatment. Cells were collected and stained with Annexin V-FITC and propidium iodide, followed by FACS analysis. Percentage population of early apoptosis (lower right quadrant) and late apoptosis (upper right quadrant) in celecoxib-treated U87MG and U87MG-E6 cells, stained with Annexin-FITC and propidium iodide are shown (A). Summary quantitative analyses of total apoptosis population following celecoxib treatment are presented (B-C). Values are expressed as mean ± S.E.M., n = 3–6. *P < 0.05, significantly different from respective controls (1-way ANOVA, Dunnett's test). #P < 0.05, significantly different between PFT-treated and -untreated U87MG cells (Mann-Whitney U-test).

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Molecular Cancer

ISSN: 1476-4598