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Figure 5 | Molecular Cancer

Figure 5

From: Docetaxel-induced prostate cancer cell death involves concomitant activation of caspase and lysosomal pathways and is attenuated by LEDGF/p75

Figure 5

Overexpression of LEDGF/p75 attenuates DTX-induced cell death. A. LEDGF/p75 expression in parental PC3 cells, pools of PC3 clones stably transfected with empty pcDNA vector (Vec), or pcDNA-ledgfp75 (p75), analyzed by immunoblotting (right panel) and RT-PCR: B. Stable overexpression of LEDGF/p75 attenuated DTX-induced cytotoxicity after 36 and 48 h of exposure to low and high concentrations of the drug. Cell survival was determined by crystal violet staining. Data is representative of three independent experiments. Error bars represent the standard deviation of at least three independent experiments done in triplicates (* p < 0.05, t-test): C. Morphology of PC3 clones (Vec and p75) treated with DTX for 48 hours. Clones overexpressing LEDGF/p75 showed less dead and detached cells than clones transfected with empty vector: D. Cell cycle analysis of PC3 clones (Vec, and p75) treated with DTX for 48 h. Flow cytometric analysis of cells stained with propidium iodide was used to determine the percentage of subG1 cells. Overexpression of LEDGF/p75 significantly attenuated DTX-induced DNA fragmentation. Representative DNA histograms are shown. Error bars represent the standard deviation of at least three independent experiments done in triplicates (* p < 0.05, t-test).

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