Overexpression of LEDGF/p75 attenuates DTX-induced LMP. PC3 cell clones (Vec and p75) were treated with DTX for up to 48 hours and stained with acridine orange (AO) to visualize LMP. Clones stably transfected with empty vector (Vec) showed increased LMP as indicated by minimal or absence of punctuated lysosomal red fluorescence, associated with increased number of detached yellow cells. Clones overexpressing LEDGF/p75 showed less cell detachment and increased lysosomal stability after DTX treatment. These resistant clones displayed extensive multinucleation.