The growth inhibition of Capan-2 cells by ATRA was accompanied by an increase in the protein level of Chmp1A, CRBP-1, P53, and phospho-P53. (A) Capan-2 cells were counted and equal numbers of cells were seeded in 10 cm tissue culture dishes. Next day, cells were treated with control DMSO or ATRA. The following 5 Days, cells were counted and plotted for the graph. Dashed line with square represents DMSO treated and straight line with circle represents ATRA treated. On Day 1 the cells did not show any difference in growth. However, the growth was inhibited significantly (based on P-value) by ATRA treatment from Day 2 on. (B, C) The cells were processed for Western blot analysis following lysis. Notice the increase of protein level of Chmp1A, CRBP-1, P53 and phospho-P53 at serine 15 and 37 upon ATRA treatment.