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Table 6 Primers used for quantitative real-time PCR

From: Gene expression profiling of canine osteosarcoma reveals genes associated with short and long survival times

Gene symbol Forward and Reverse Primers Temp (°C)
ANKRD17 FW: 5'-AAGTAGCGCACCACCTTCAC-3' 60.0
  RW: 5'-CTAGCAGCAAATGGTGGACA-3'  
MRPS31 FW: 5'-GAATTGGTCCTTGCTTTGGA-3' 60.0
  RW: 5'-ATCCAGTGGACGAAAGATGG-3'  
NCOR1 FW: 5'-TCTTCCTCTGCGTTTTCCAT-3' 59.6
  RW: 5'-GCATCCCAAAAACTTTGGAC-3'  
MGST1 FW: 5'-CGGACAGATGATAGGGTGG-3' 62.0
  RW: 5'-GATTTGGCTGGGGAAGG-3'  
RPS19 FW: 5'-CCTTCCTCAAAAAGTCTGGG-3' 61.0
  RW: 5'-GTTCTCATCGTAGGGAGCAAG-3'  
HPRT FW: 5'-AGCTTGCTGGTGAAAAGGAC-3' 56.0
  RW: 5'-TTATAGTCAAGGGCATATCC-3'